DNA isolation from fresh and cryopreserved semen of Tambaqui (Colossoma macropomum) - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Journal of FisheriesSciences.com Year : 2016

DNA isolation from fresh and cryopreserved semen of Tambaqui (Colossoma macropomum)

Abstract

Semen cryopreservation is an import toll for reproductive management of fish species, which increasingly it is applied in the commercial production of fish. Cryopreservation need of the cryoprotectors agents (CPAs) that may be a complication factor in DNA extraction that can affect the quality of isolated DNA. Genomic DNA constitutes the total genetic information of an organism and it is used for a many finalities, such as to determine gene structure or to detect the presence or absence of genes of interest. However, simple questions to be analyzed become slow to be answered due to troubles in the execution of basic techniques like DNA isolation. This article describes a protocol for the isolation of genomic DNA from fresh and cryopreserved semen.
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Dates and versions

hal-02633969 , version 1 (27-05-2020)

Identifiers

  • HAL Id : hal-02633969 , version 1
  • PRODINRA : 371628

Cite

Fernanda de Mello, Juliana Saraiva Garcia, Alexandra Depince, Catherine Labbé, Danilo Pedro Jr. Streit. DNA isolation from fresh and cryopreserved semen of Tambaqui (Colossoma macropomum). Journal of FisheriesSciences.com, 2016, 10 (2), pp.21-24. ⟨hal-02633969⟩
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