Both Structural and Non-Structural Forms of the Readthrough Protein of Cucurbit aphid-borne yellows virus Are Essential for Efficient Systemic Infection of Plants
Résumé
Cucurbit aphid-borne yellows virus (CABYV) is a polerovirus (Luteoviridae family) with a capsid composed of the major coat protein and a minor component referred to as the readthrough protein (RT). Two forms of the RT were reported: a full-length protein of 74 kDa detected in infected plants and a truncated form of 55 kDa (RT*) incorporated into virions. Both forms were detected in CABYV-infected plants. To clarify the specific roles of each protein in the viral cycle, we generated by deletion a polerovirus mutant able to synthesize only the RT* which is incorporated into the particle. This mutant was unable to move systemically from inoculated leaves inferring that the C-terminal half of the RT is required for efficient long-distance transport of CABYV. Among a collection of CABYV mutants bearing point mutations in the central domain of the RT, we obtained a mutant impaired in the correct processing of the RT which does not produce the RT*. This mutant accumulated very poorly in upper non-inoculated leaves, suggesting that the RT* has a functional role in long-distance movement of CABYV. Taken together, these results infer that both RT proteins are required for an efficient CABYV movement.
Fichier principal
Boissinot_PlosOne_2014_1.pdf (2.46 Mo)
Télécharger le fichier
Figure_S1.tif (229.82 Ko)
Télécharger le fichier
Figure_S2.tif (195.39 Ko)
Télécharger le fichier
Figure_S3.tif (258.04 Ko)
Télécharger le fichier
Figure_S4.tif (309.61 Ko)
Télécharger le fichier
Figure_S5.tif (312.74 Ko)
Télécharger le fichier
Figure_S6.tif (255.87 Ko)
Télécharger le fichier
Figure_S7.tif (709.9 Ko)
Télécharger le fichier
Figure_S8.tif (174 Ko)
Télécharger le fichier
Figure_S9.tif (600.32 Ko)
Télécharger le fichier
Table_S1.tif (210.47 Ko)
Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte