Regulation of a maize HD-ZIP IV transcription factor by a non-conventional RDR2-dependent small RNA. - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Plant Journal Year : 2015

Regulation of a maize HD-ZIP IV transcription factor by a non-conventional RDR2-dependent small RNA.

Abstract

Small non-coding RNAs are versatile riboregulators that control gene expression at the transcriptional or post-transcriptional level, governing many facets of plant development. Here we present evidence for the existence of a 24 nt small RNA (named small1) that is complementary to the 3' UTR of OCL1 (Outer Cell Layer1), the founding member of the maize HD-ZIP IV gene family encoding plant-specific transcription factors that are mainly involved in epidermis differentiation and specialization. The biogenesis of small1 depends on DICER-like 3 (DCL3), RNA-dependent RNA polymerase 2 (RDR2) and RNA polymerase IV, components that are usually required for RNA-dependent DNA-methylation. Unexpectedly, GFP sensor experiments in transient and stable transformation systems revealed that small1 may regulate its target at the post-transcriptional level, mainly through translational repression. This translational repression is attenuated in an rdr2 mutant background in which small1 does not accumulate. Our experiments further showed the possible involvement of a secondary stem-loop structure present in the 3' UTR of OCL1 for efficient target repression, suggesting the existence of several regulatory mechanisms affecting OCL1 mRNA stability and translation.

Dates and versions

hal-02634338 , version 1 (27-05-2020)

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Catherine Klein-Cosson, Pierre Chambrier, Peter Rogowsky, Vanessa Vernoud. Regulation of a maize HD-ZIP IV transcription factor by a non-conventional RDR2-dependent small RNA.. Plant Journal, 2015, 81 (5), pp.747-758. ⟨10.1111/tpj.12771⟩. ⟨hal-02634338⟩
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