Structural alteration of cell wall pectins accompanies pea development in response to cold - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement
Journal Articles Phytochemistry Year : 2014

Structural alteration of cell wall pectins accompanies pea development in response to cold

Abstract

Pea (Pisum sativum) cell wall metabolism in response to chilling was investigated in a frost-sensitive genotype 'Terese' and a frost-tolerant genotype 'Champagne'. Cell walls isolated from stipules of cold acclimated and non-acclimated plants showed that cold temperatures induce changes in polymers containing xylose, arabinose, galactose and galacturonic acid residues. In the tolerant cultivar Champagne, acclimation is accompanied by increases in homogalacturonan, xylogalacturonan and highly branched Rhamnogalacturonan I with branched and unbranched (1 -> 5)-alpha-arabinans and (1 -> 4)-beta-galactans. In contrast, the sensitive cultivar Terese accumulates substantial amounts of (1 -> 4)-beta-xylans and glucuronoxylan, but not the pectins. Greater JIM7 labeling was observed in Champagne compared to Terese, indicating that cold acclimation also induces an increase in the degree of methylesterification of pectins. Significant decrease in polygalacturonase activities in both genotypes were observed at the end of cold acclimation. These data indicate a role for esterified pectins in cold tolerance. The possible functions for pectins and their associated arabinans and galactans in cold acclimation are discussed.
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Dates and versions

hal-02636560 , version 1 (27-05-2020)

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Laetitia Baldwin, Jean-Marc Domon, John F. Klimek, Françoise Fournet, Hélène Sellier, et al.. Structural alteration of cell wall pectins accompanies pea development in response to cold. Phytochemistry, 2014, 104, pp.37-47. ⟨10.1016/j.phytochem.2014.04.011⟩. ⟨hal-02636560⟩
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