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Quantifying hydrostatic pressure in plant cells by using Indentation with an atomic force microscope

Abstract : Plant cell growth depends on a delicate balance between an inner drive-the hydrostatic pressure known as turgor-and an outer restraint-the polymeric wall that surrounds a cell. The classical technique to measure turgor in a single cell, the pressure probe, is intrusive and cannot be applied to small cells. In order to overcome these limitations, we developed a method that combines quantification of topography, nanoindentation force measurements, and an interpretation using a published mechanical model for the pointlike loading of thin elastic shells. We used atomic force microscopy to estimate the elastic properties of the cell wall and turgor pressure from a single force-depth curve. We applied this method to onion epidermal peels and quantified the response to changes in osmolality of the bathing solution. Overall our approach is accessible and enables a straightforward estimation of the hydrostatic pressure inside a walled cell.
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Submitted on : Wednesday, May 27, 2020 - 9:27:12 PM
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Léna Beauzamy, Julien Derr, Arezki Boudaoud. Quantifying hydrostatic pressure in plant cells by using Indentation with an atomic force microscope. Biophysical Journal, Biophysical Society, 2015, 108 (10), pp.2448-2456. ⟨10.1016/j.bpj.2015.03.035⟩. ⟨hal-02636803⟩

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