Skip to Main content Skip to Navigation
Journal articles

Transcriptional responses of PBMC in psychosocially stressed animals indicate an alerting of the immune system in female but not in castrated male pigs

Abstract : Background Brain and immune system are linked in a bi-directional manner. To date, it remained largely unknown why immune components become suppressed, enhanced, or remain unaffected in relation to psychosocial stress. Therefore, we mixed unfamiliar pigs with different levels of aggressiveness. We separated castrated male and female pigs into psychosocially high- and low- stressed animals by skin lesions, plasma cortisol level, and creatine kinase activity obtained from agonistic behaviour associated with regrouping. Peripheral blood mononuclear cells (PBMC) were collected post-mortem and differential gene expression was assessed using the Affymetrix platform (n = 16). Results Relevant stress-dependent alterations were found only between female samples, but not between castrated male samples. Molecular routes related to TREM 1 signalling, dendritic cell maturation, IL-6 signalling, Toll-like receptor signalling, and IL-8 signalling were increased in high stressed females compared to low stressed females. This indicates a launch of immune effector molecules as a direct response. According to the shifts of transcripts encoding cell surface receptors (e.g. CD14, TLR2, TLR4, TREM1) the study highlights processes acting on pattern recognition, inflammation, and cell-cell communication. Conclusions The transcriptional response partly affected the degree of ‘stress responsiveness’, indicating that the high stressed females altered their signal transduction due to potential infections and injuries while fighting.
Document type :
Journal articles
Complete list of metadata

Cited literature [43 references]  Display  Hide  Download

https://hal.inrae.fr/hal-02638885
Contributor : Migration Prodinra <>
Submitted on : Thursday, May 28, 2020 - 11:01:58 AM
Last modification on : Tuesday, July 20, 2021 - 4:06:31 AM

File

2014_Oster_BMCGenomics_1.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Michael Oster, Eduard Muráni, Siriluck Ponsuksili, Richard B. d'Eath, Simon P. Turner, et al.. Transcriptional responses of PBMC in psychosocially stressed animals indicate an alerting of the immune system in female but not in castrated male pigs. BMC Genomics, BioMed Central, 2014, 15, pp.1-11. ⟨10.1186/1471-2164-15-967⟩. ⟨hal-02638885⟩

Share

Metrics

Record views

25

Files downloads

50