Sporulation temperature reveals a requirement for CotE in the assembly of both the coat and exosporium layers of Bacillus cereus spores - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Applied and Environmental Microbiology Year : 2016

Sporulation temperature reveals a requirement for CotE in the assembly of both the coat and exosporium layers of Bacillus cereus spores

Abstract

The Bacillus cereus spore surface layers consist of a coat surrounded by an exosporium. We investigated the interplay between the sporulation temperature and the CotE morphogenetic protein in the assembly of the surface layers of B. cereus ATCC14579 spores and on the resulting spore properties. The cotE deletion affects the coat and exosporium composition of the spores formed at both the suboptimal temperature of 20°C and at the optimal growth temperature of 37°C. Transmission electron microscopy revealed that ΔcotE spores had a fragmented and detached exosporium when formed at 37°C. However, when produced at 20°C, ΔcotE spores showed defects in both coat and exosporium attachment, and were susceptible to lysozyme and mutanolysin. Thus, CotE has a role in the assembly of both the coat and exosporium, more important during sporulation at 20°C. CotE was more represented in extracts from spores formed at 20°C, suggesting that increased synthesis of the protein is required to maintain proper assembly of spore surface layers at this temperature. ΔcotE spores formed at either sporulation temperature were impaired in inosine-triggered germination, resistance to UV-C and H2O2, and were less hydrophobic than WT spores, but had a higher resistance to wet-heat. While underscoring the role of CotE in the assembly of B. cereus spore surface layers, our study also suggests a contribution of the protein to functional properties of additional spore structures. Moreover, it also suggests a complex relationship between the function of a spore morphogenetic protein and environmental factors such as the temperature during spore formation.

Dates and versions

hal-02641494 , version 1 (28-05-2020)

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Christelle Bressuire-Isoard, Isabelle Bornard, Adriano O. Henriques, Frederic F. Carlin, Veronique V. Broussolle. Sporulation temperature reveals a requirement for CotE in the assembly of both the coat and exosporium layers of Bacillus cereus spores. Applied and Environmental Microbiology, 2016, 82 (1), pp.232-243. ⟨10.1128/AEM.02626-15⟩. ⟨hal-02641494⟩
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