Specificity of IgE antibodies from patients allergic to goat's milk and tolerant to cow's milk determined with plasmin-derived peptides of bovine and caprine β-caseins - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement
Journal Articles Molecular Nutrition and Food Research Year : 2012

Specificity of IgE antibodies from patients allergic to goat's milk and tolerant to cow's milk determined with plasmin-derived peptides of bovine and caprine β-caseins

Abstract

Scope Despite a sequence homology of 90% between bovine and caprine beta-caseins (CN), IgE antibodies from patients allergic to goat's milk (GM), but tolerant to cow's milk (CM), recognize caprine beta-CN without cross-reacting with bovine beta-CN. We investigated this lack of cross-reactivity by evaluating the IgE-reactivity toward peptides isolated from plasmin hydolysates of bovine and caprine beta-CN. Methods and results The IgE-binding capacity of plasmin-derived peptides was evaluated with sera from 10 CM-allergic patients and 12 GM-allergic/CM-tolerant patients. In CM-allergic patients, IgE reactivity of caprine fragments (f29-107) and (f108-207), but not (f1-28), was similar to that of the bovine counterparts. In contrast, all bovine fragments were poorly recognized by IgE antibodies from GM-allergic/CM-tolerant patients. The peptide (f29-107) was generally the most immunoreactive fragment of caprine beta-CN. By using synthetic peptides, the immunodominant IgE-binding epitope recognized by most GM-allergic/CM-tolerant patients was located in the caprine domain 4979. Conclusion The restricted specificity of the IgE response toward the caprine beta-CN in GM-allergic/CM-tolerant patients is mainly directed against the domain 4979, which differs from its bovine counterpart by only three amino acid substitutions.
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hal-02648963 , version 1 (01-09-2023)

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Hervé Bernard, Sandrine Ah-Leung-Poullias, Sylvain Tilleul, Marie-Francoise Drumare, Evelyne Paty, et al.. Specificity of IgE antibodies from patients allergic to goat's milk and tolerant to cow's milk determined with plasmin-derived peptides of bovine and caprine β-caseins. Molecular Nutrition and Food Research, 2012, 56 (10), pp.1532-1540. ⟨10.1002/mnfr.201200229⟩. ⟨hal-02648963⟩
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