Lycopene attenuates LPS-induced TNF-alpha secretion in macrophages and inflammatory markers in adipocytes exposed to macrophage-conditioned media - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Molecular Nutrition and Food Research Year : 2012

Lycopene attenuates LPS-induced TNF-alpha secretion in macrophages and inflammatory markers in adipocytes exposed to macrophage-conditioned media

Julie Marcotorchino
  • Function : Author
Beatrice Romier
  • Function : Author
Erwan Gouranton
  • Function : Author
Céline Riollet
  • Function : Author
Béatrice Gleize
Christiane Malezet-Desmoulins
  • Function : Author

Abstract

Scope Adipose tissue is infiltrated by an increasing number of macrophages during the development of obesity. These immune cells are suspected to be a major source of TNF-a that interferes with adipocyte function. Because lycopene possesses anti-inflammatory properties, we hypothesize that lycopene could reduce the production of TNF-a by macrophages and thus interfere in the cross-talk between macrophages and adipocytes. Methods and results We demonstrated that physiological concentrations of lycopene were able to attenuate the lipopolysaccharide (LPS)-mediated induction of TNF-a in RAW 264.7 macrophages, at both the mRNA and protein levels. The molecular mechanism was studied. It appeared that the LPS-activation of both JNK and NF-?B signaling pathways was modulated by lycopene. The anti-inflammatory effects of lycopene on macrophages were accompanied by a decrease in LPS-stimulated macrophage migration in the presence of lycopene. Furthermore, lycopene decreased macrophage conditioned medium-induced proinflammatory cytokine, acute phase protein, and chemokine mRNA expression in 3T3-L1 adipocytes. Conclusion These data indicate that lycopene displayed an anti-inflammatory effect on macrophages that beneficially impacted adipocyte function. Thus, these results suggest that lycopene could block the vicious cycle that occurs between adipocytes and macrophages in adipose tissue during obesity.

Dates and versions

hal-02649163 , version 1 (29-05-2020)

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Julie Marcotorchino, Beatrice Romier, Erwan Gouranton, Céline Riollet, Béatrice Gleize, et al.. Lycopene attenuates LPS-induced TNF-alpha secretion in macrophages and inflammatory markers in adipocytes exposed to macrophage-conditioned media. Molecular Nutrition and Food Research, 2012, 56 (5), pp.725 - 732. ⟨10.1002/mnfr.201100623⟩. ⟨hal-02649163⟩
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