Skip to Main content Skip to Navigation
Journal articles

Abscisic acid effects on activity and expression of barley (Hordeum vulgare) plastidial glucose-6-phosphate dehydrogenase

Abstract : Total glucose-6-phosphate dehydrogenase (G6PDH) activity, protein abundance, and transcript levels of G6PDH isoforms were measured in response to exogenous abscisic acid (ABA) supply to barley (Hordeum vulgare cv Nure) hydroponic culture. Total G6PDH activity increased by 50% in roots treated for 12 h with exogenous 0.1 mM ABA. In roots, a considerable increase (35%) in plastidial P2-G6PDH transcript levels was observed during the first 3 h of ABA treatment. Similar protein variations were observed in immunoblotting analyses. In leaves, a 2-fold increase in total G6PDH activity was observed after ABA treatment, probably related to an increase in the mRNA level (increased by 50%) and amount of protein (increased by 85%) of P2-G6PDH. Together these results suggest that the plastidial P2-isoform plays an important role in ABA-treated barley plants.
Document type :
Journal articles
Complete list of metadata

https://hal.inrae.fr/hal-02649210
Contributor : Migration Prodinra Connect in order to contact the contributor
Submitted on : Friday, May 29, 2020 - 11:33:15 AM
Last modification on : Wednesday, September 22, 2021 - 1:46:04 PM

Links full text

Identifiers

Collections

Citation

Manuela Cardi, Kamel Chibani, Donata Cafasso, Nicolas Rouhier, Jean-Pierre Jacquot, et al.. Abscisic acid effects on activity and expression of barley (Hordeum vulgare) plastidial glucose-6-phosphate dehydrogenase. Journal of Experimental Botany, Oxford University Press (OUP), 2011, 62 (11), pp.4013 - 4023. ⟨10.1093/jxb/err100⟩. ⟨hal-02649210⟩

Share

Metrics

Record views

16