Functional insights from the GC-poor genomes of two aphid parasitoids, Aphidius ervi and Lysiphlebus fabarum

Alice Dennis; Gabriel Ballesteros; Stéphanie Robin; Lukas Schrader; Jens Bast; Jan Berghöfer; Leo Beukeboom; Maya Belghazi; Anthony Bretaudeau; Jan Buellesbach; Elizabeth Cash; Dominique Colinet; Zoé Dumas; Mohammed Errbii; Patrizia Falabella; Jean-Luc Gatti; Elzemiek Geuverink; Joshua Gibson; Corinne Hertaeg; Stefanie Hartmann; Emmanuelle Jacquin-Joly; Mark Lammers; Blas Lavandero; Ina Lindenbaum; Lauriane Massardier-Galatà; Camille Meslin; Nicolas Montagné; Nina Pak; Marylène Poirié; Rosanna Salvia; Chris Smith; Denis Tagu; Sophie Tares; Heiko Vogel; Tanja Schwander; Jean-Christophe Simon; Christian Figueroa; Christoph Vorburger; Fabrice Legeai; Jürgen Gadau

doi:10.1186/s12864-020-6764-0

Article dans une revue

Functional insights from the GC-poor genomes of two aphid parasitoids, Aphidius ervi and Lysiphlebus fabarum

Alice Dennis ^{1, *} Gabriel Ballesteros ² Stéphanie Robin ³ Lukas Schrader ⁴ Jens Bast ⁵ Jan Berghöfer ⁴ Leo Beukeboom ⁶ Maya Belghazi ⁷ Anthony Bretaudeau ³ Jan Buellesbach ⁴ Elizabeth Cash ⁸ Dominique Colinet ⁹ Zoé Dumas ⁵ Mohammed Errbii ⁴ Patrizia Falabella ^{10, 11} Jean-Luc Gatti ⁹ Elzemiek Geuverink ⁶ Joshua Gibson ^{12, 13} Corinne Hertaeg ¹ Stefanie Hartmann ¹⁴ Emmanuelle Jacquin-Joly ¹⁵ Mark Lammers ¹³ Blas Lavandero ² Ina Lindenbaum ⁴ Lauriane Massardier-Galatà ⁹ Camille Meslin ¹⁶ Nicolas Montagné ¹⁵ Nina Pak ⁸ Marylène Poirié ⁹ Rosanna Salvia ¹⁰ Chris Smith ¹⁷ Denis Tagu ¹¹ Sophie Tares ⁹ Heiko Vogel ¹⁸ Tanja Schwander ⁵ Jean-Christophe Simon ¹¹ Christian Figueroa ² Christoph Vorburger ¹⁰ Fabrice Legeai ¹¹ Jürgen Gadau ^{19, *}

* Auteur correspondant

1 Department of Aquatic Ecology - (Dübendorf, Suisse)

2 Universidad de Talca

3 GEF - Plateforme Génomique Environnementale et Fonctionnelle

4 IEB - Institute for Evolution and Biodiversity

5 DEE - Département d'écologie et évolution [Lausanne]

6 GELIFES - Groningen Institute for Evolutionary Life Sciences [Groningen]

7 INP - Institut de neurophysiopathologie

8 Department of Environmental Sciences [Riverside]

9 ISA - Institut Sophia Agrobiotech

10 UNIBAS - Università degli studi della Basilicata [Potenza]

11 IGEPP - Institut de Génétique, Environnement et Protection des Plantes

12 Georgia Southern University

13 University of California [Berkeley]

14 Universität Potsdam

15 PISC - Physiologie de l'Insecte, Signalisation et Communication [Versailles]

16 IEES (UMR_7618 / UMR_D_242 / UMR_A_1392 / UM_113) - Institut d'écologie et des sciences de l'environnement de Paris

17 CRISCO - Centre de recherche inter-langues sur la signification en contexte

18 Max Planck Institute for Chemical Ecology

19 ASU - Arizona State University [Tempe]

Abstract : Background: Parasitoid wasps have fascinating life cycles and play an important role in trophic networks, yet little is known about their genome content and function. Parasitoids that infect aphids are an important group with the potential for biological control. Their success depends on adapting to develop inside aphids and overcoming both host aphid defenses and their protective endosymbionts.Results: We present the de novo genome assemblies, detailed annotation, and comparative analysis of two closely related parasitoid wasps that target pest aphids: Aphidius ervi and Lysiphlebus fabarum (Hymenoptera: Braconidae: Aphidiinae). The genomes are small (139 and 141 Mbp) and the most AT-rich reported thus far for any arthropod (GC content: 25.8 and 23.8%). This nucleotide bias is accompanied by skewed codon usage and is stronger in genes with adult-biased expression. AT-richness may be the consequence of reduced genome size, a near absence of DNA methylation, and energy efficiency. We identify missing desaturase genes, whose absence may underlie mimicry in the cuticular hydrocarbon profile of L. fabarum. We highlight key gene groups including those underlying venom composition, chemosensory perception, and sex determination, as well as potential losses in immune pathway genes.Conclusions: These findings are of fundamental interest for insect evolution and biological control applications. They provide a strong foundation for further functional studies into coevolution between parasitoids and their hosts. Both genomes are available at https://bipaa.genouest.org.

Keywords : DNA methylation loss GC content Lysiphlebus fabarum Parasitoid wasp Toll and Imd pathways Venom proteins de novo genome assembly. Aphid host Aphidius ervi Chemosensory genes

Type de document :

Article dans une revue

Domaine :

Sciences du Vivant [q-bio]

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https://hal.inrae.fr/hal-02649295
Déposant : Laurent Jonchère <>
Soumis le : lundi 28 septembre 2020 - 16:51:19
Dernière modification le : mercredi 20 janvier 2021 - 10:32:12

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