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Microenvironnement tubaire : rôle dans la maturation des ovocytes canins in vivo et in vitro

Abstract : In most mammals, oocytes are ovulated at the metaphase II stage, and the meiosis inhibition is then lifted by fertilization. In bitches and other Canidae species however, oocytes are released at the prophase I stage, and another 48 to 72h are necessary for them to mature into themetaphase II stage and become fertilizable. This specificity is currently hindering the development of reproductive biotechnologies in these species. In vitro maturation rates of canine oocytes are very low, as only 10 to 30%will reach the metaphase stage after 72h in culture. In bitches, nuclear maturation occurs in the oviduct, and tubal derivatives (culture media, such as Synthetic Oviductal Fluid, oviductal explants, coculture on tubal cell layers) were used to improve the yield, but so far not very successfully. This failure may be due to the lack of data on the composition of oviductal fluid in bitches. Further studies on the oviductal microenvironment of bitches are therefore necessary, as it is probably quite different from the oviductal microenvironment of other females, e.g. the presence of preovulatory luteinisation in bitches only. Creating a maturation medium based on the composition of oviductal fluid could be an interesting avenue to explore to improve in vitro maturation rates.
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Emmanuel Fontaine, Karine Reynaud, Sandra Thoumire, Martine Chebrout, Laetitia Faure, et al.. Microenvironnement tubaire : rôle dans la maturation des ovocytes canins in vivo et in vitro. Bulletin de l'Académie Vétérinaire de France, Académie vétérinaire de France, 2009, 162 (2), pp.145-154. ⟨10.4267/2042/47987⟩. ⟨hal-02653383⟩

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