Flavonoids and their oxidation products protect efficiently albumin-bound linoleic acid in a model of plasma oxidation - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Biochimica et Biophysica Acta (BBA) - General Subjects Year : 2007

Flavonoids and their oxidation products protect efficiently albumin-bound linoleic acid in a model of plasma oxidation

Abstract

Although LDL esterified polyunsaturated fatty acids (PUFA) contribute largely to the pool of oxidizable lipids in plasma, they coexist with a non-negligible content of free PUFA. In some pathological conditions, the free PUFA/albumin ratio becomes abnormally elevated. Modeling was performed in a system constituted of linoleic acid bound to human serum albumin (HSA) in which oxidation was initiated by hydrophilic AAPH. Inhibition of lipid peroxidation was evaluated for various flavonoids. The accumulations of hydroperoxyoctadecadienoic acids (HPODE), hydroxyoctadecadienoic acids (HODE) and ketooctadecadienoic acids (KODE) were similarly inhibited: isoquercitrin>quercetin>catechin=isorhamnetin>>kaempferol>quercetin-4'-beta-D-glucoside=quercetin-3,4'-di-beta-D-glucoside. Surprisingly, quercetin and isorhamnetin afforded a protection to linoleic acid long after their consumption. Elucidation by mass spectrometry and NMR of the quercetin oxidation products and assessment of their antioxidant capacity pointed out that 3,4-dihydroxybenzoic acid and 2-(3,4-dihydroxybenzoyl)-2,4,6-trihydroxybenzofuran-3(2H)-one are major contributors to the apparent quercetin antioxidant capacity.

Dates and versions

hal-02655355 , version 1 (29-05-2020)

Identifiers

Cite

Claire Dufour, Michele Loonis. Flavonoids and their oxidation products protect efficiently albumin-bound linoleic acid in a model of plasma oxidation. Biochimica et Biophysica Acta (BBA) - General Subjects, 2007, 1770 (6), pp.958-965. ⟨10.1016/j.bbagen.2007.02.005⟩. ⟨hal-02655355⟩
8 View
0 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More