In plant tissues, xenobiotics often are conjugated with natural constituents such as sugars, amino acids, glutathione, and malonic acid. Usually, conjugation processes result in a decrease in the reactivity and toxicity of xenobiotics by increasing the water solubility and polarity of conjugates, and reducing their mobility. Due to their lack of an efficient excretory system, xenobiotic conjugates finally are sequestered in plant storage compartments or cell vacuoles, or are integrated as bound residues in cell walls. Chlorophenols are potentially harmful pollutants that are found in numerous natural and agricultural systems. we studied the metabolic fate of 2,4‐dichlorophenol (DCP) in cell‐suspension cultures of tobacco (Nicotiana tabacum L.). After a standard metabolism experiment, 48h of incubation with a [U‐phenyl‐14C]‐DCP solution, aqueous extracts of cell suspension cultures were analyzed by high‐performance liquid chromatography (HPLC). Metabolites then were isolated and their chemical structures determined by enzymatic and chemical hydrolyses, electrospray ionization‐mass spectrometry in negative mode (ESI‐NI), and 1H nuclear magnetic resonance analyses. The main terminal metabolites identified were DCP‐glycoside conjugates, DCP‐(6‐O‐malonyl)‐glucoside, DCP‐(6‐O‐acetyl)‐glucoside, and their precursor, DCP‐glucoside. More unusual and complex DCP conjugates such as an α(1→6)‐glucosyl‐pentose and a triglycoside containing a glucuronic acid were further characterized. All the metabolites identified were complex glycoside conjugates. However, these conjugates still may be a source of DCP in hydrolysis reactions caused by microorganisms in the environment or in the digestive tract of animals and humans. Removal of xenobiotics by glycoside conjugation thus may result in underestimation of the risk associated with toxic compounds like DCP in the environment or in the food chain.