Automated image analysis of bacterial colony growth as a tool to study individual lag time distributions of immobilized cells - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Journal of Microbiological Methods Year : 2006

Automated image analysis of bacterial colony growth as a tool to study individual lag time distributions of immobilized cells

Abstract

A method to determine the individual lag time (lag) distributions of immobilized bacteria was presented. The method was based on the image analysis of the bacterial colony growth. The lag distributions were retrieved from the distributions of the detection times (Td) required to form macroscopically visible colonies. Using this method, the lag distributions on agar for Listeria monocytogenes cells previously subjected to two situations reproducing conditions encountered during the contamination of cheese, were determined. The results were presented and compared with lag distributions obtained with an established method based on the time to detection of turbidity in broth. An original method to retrieve lag in broth and agar without any knowledge of the growth rate was also proposed. In order not to bias the distributions of lag on agar the impact of spatial separation between colonies on colony growth rates was quantified. Means and standard deviations of lag distributions for the two different stresses were found to be similar in broth and on agar. Extreme Value type II distributions fitted the best the different datasets of lag distributions.

Dates and versions

hal-02657976 , version 1 (30-05-2020)

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Laurent Guillier, Pierre Pardon, J.C. Augustin. Automated image analysis of bacterial colony growth as a tool to study individual lag time distributions of immobilized cells. Journal of Microbiological Methods, 2006, 65 (2), pp.324-334. ⟨10.1016/j.mimet.2005.08.007⟩. ⟨hal-02657976⟩

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