Determination of the toxic potential of Bacillus cereus isolates by quantitative enterotoxin analyses - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles FEMS Microbiology Letters Year : 2006

Determination of the toxic potential of Bacillus cereus isolates by quantitative enterotoxin analyses

Abstract

Haemolysin BL (HBL) and nonhaemolytic enterotoxin (Nhe), each consisting of three components, represent the major enterotoxins produced by Bacillus cereus. To evaluate the expression of these toxins, a set of 100 B. cereus strains was examined. Molecular biological characterization showed that 42% of the strains harboured the genes for HBL and 99% for Nhe. The production of all Nhe and HBL components were analyzed using specific antibodies and, in culture supernatants, detectable levels of HBL and Nhe were found for 100% of hbl-positive and 96% of nhe-positive strains. The concentrations of the HBL–L2 and NheB component ranged from 0.02 to 5.6 mgmL 1 and from 0.03 to 14.2 mgmL 1, respectively. Comparison of the amount of NheB produced by food poisoning and food/environmental strains revealed that the median value for all food poisoning strains was significantly higher than for the food/environmental isolates. The data presented in this study provide evidence that specific and quantitative determination of the enterotoxins is necessary to evaluate the toxic potential of B. cereus. In particular, the level of Nhe seems to explain most of the cytotoxic activity of B. cereus isolates and may indicate a highly diarrheic potential.

Dates and versions

hal-02660892 , version 1 (30-05-2020)

Identifiers

Cite

Maximilian Moraveek, Richard Dietrich, Christine Buerk, Veronique V. Broussolle, Marie Helene M. H. Guinebretière, et al.. Determination of the toxic potential of Bacillus cereus isolates by quantitative enterotoxin analyses. FEMS Microbiology Letters, 2006, 257, pp.293-298. ⟨10.1111/j.1574-6968.2006.00185.x⟩. ⟨hal-02660892⟩
16 View
0 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More