Skip to Main content Skip to Navigation
Journal articles

Glycine betaine improves Listeria monocytogenes tolerance to desiccation on parsley leaves independent of the osmolyte transporters BetL, Gbu and OpuC

Abstract : Aims: To investigate the effect of glycine betaine (GB) on the survival of Listeria monocytogenes on leaf surfaces under low relative humidity (RH). Methods and Results: The addition of GB (≥25 mmol l−1) improved the survival of L. monocytogenes under low RH on parsley leaves, thus suggesting that GB can improve the tolerance of L. monocytogenes to desiccation. Ten times less GB was needed to improve L. monocytogenes survival under low RH on nonbiological surfaces compared with parsley leaves, suggesting that, on the leaf surface, L. monocytogenes may have to compete for the available GB with autochthonous bacteria and/or the plant itself. Wild type and mutants carrying deletions in the three GB uptake systems, BetL, Gbu and OpuC, behaved similarly with and without added GB on parsley leaves (P > 0·05). In addition, preaccumulation of GB, triggered by osmotic stress prior to inoculation, failed to improve survival under low RH compared with osmotic stress without GB accumulation. Conclusions: Exogenous GB had a protective effect on L. monocytogenes cells from desiccation during survival on parsley leaves. This effect was independent of intracellular GB accumulation by the known uptake systems. Significance and Impact of the Study: Presence of GB could improve the survival of L. monocytogenes to desiccation on leaf surfaces and nonbiological surfaces
Document type :
Journal articles
Complete list of metadata

https://hal.inrae.fr/hal-02661159
Contributor : Migration Prodinra <>
Submitted on : Saturday, May 30, 2020 - 9:48:14 PM
Last modification on : Tuesday, April 6, 2021 - 4:38:45 PM

Links full text

Identifiers

Collections

Citation

Nicolas Dreux, Guy Albagnac, R.D. Sleator, C. Hill, Frederic Carlin, et al.. Glycine betaine improves Listeria monocytogenes tolerance to desiccation on parsley leaves independent of the osmolyte transporters BetL, Gbu and OpuC. Journal of Applied Microbiology, Wiley, 2008, 104 (4), pp.1221-1227. ⟨10.1111/j.1365-2672.2007.03623.x⟩. ⟨hal-02661159⟩

Share

Metrics

Record views

23