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Journal Articles Journal of Applied Microbiology Year : 2008

Response of listeria monocytogenes to liquid smoke

Abstract

Aims: To investigate the effect of liquid smoke on growth, survival, proteomic pattern and haemolytic potential of Listeria monocytogenes. Methods and Results: Growth and survival curves were recorded in brain-heart infusion broth supplemented with three concentrations of liquid smoke. L. monocytogenes growth was inhibited in the presence of 15 mu g ml(-1) phenol while a rapid decrease in cell viability occurred in the presence of 30 mu g ml(-1) phenol. The proteome of L. monocytogenes cytosoluble proteins was slightly modified after 2-h incubation with 30 mu g ml(-1) phenol but no protein already characterized in response to other known stresses was induced, except the protease ClpP. Liquid smoke inhibited the haemolytic potential without affecting hly gene expression, showing a potential inhibition of protein activity or stability. Conclusions: The presence of liquid smoke in a rich medium strongly affected growth and survival of L. monocytogenes. Brief smoke stress affected the metabolic pathways and inhibited the haemolytic activity of L. monocytogenes.

Dates and versions

hal-02662626 , version 1 (31-05-2020)

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Morgan M. Guilbaud, Ingrid Chafsey, Marie-France M.-F. Pilet, Françoise Leroi, Herve H. Prévost, et al.. Response of listeria monocytogenes to liquid smoke. Journal of Applied Microbiology, 2008, 104 (6), pp.1744-1753. ⟨10.1111/j.1365-2672.2008.03731.x⟩. ⟨hal-02662626⟩
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