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Journal Articles Genome Biology Year : 2008

Annotating genomes with massive-scale RNA sequencing

Patrick Wincker

Abstract

Next generation technologies enable massive-scale cDNA sequencing (so-called RNA-Seq). Mainly because of the difficulty of aligning short reads on exon-exon junctions, no attempts have been made so far to use RNA-Seq for building gene models de novo, that is, in the absence of a set of known genes and/or splicing events. We present G-Mo.R-Se (Gene Modelling using RNA-Seq), an approach aimed at building gene models directly from RNA-Seq and demonstrate its utility on the grapevine genome.

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Biotechnology
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Dates and versions

hal-02664963 , version 1 (31-05-2020)

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France Denoeud, Jean-Marc Aury, Corinne da Silva, Benjamin Noel, Odile Rogier, et al.. Annotating genomes with massive-scale RNA sequencing. Genome Biology, 2008, 9 (12), ⟨10.1186/gb-2008-9-12-r175⟩. ⟨hal-02664963⟩
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