Proteome analysis of the sarcoplasmic fraction of pig semimembranosus muscle: implication on meat color development - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Journal of Agricultural and Food Chemistry Year : 2006

Proteome analysis of the sarcoplasmic fraction of pig semimembranosus muscle: implication on meat color development

Abstract

Two-dimensional electrophoresis was used to investigate sarcoplasmic protein expression in pig Semimembranosus muscles sampled 20 min after slaughter. Two groups (light and dark) of 12 animals were selected from 1000 pigs, based on meat L* values measured 36 h postmortem. Twenty-two proteins or fragments (p < 0.05) were differentially expressed. Muscles leading to darker meat had a more oxidative metabolism, indicated by more abundant mitochondrial enzymes of the respiratory chain, hemoglobin, and chaperone or regulator proteins (HSP27, alpha B-crystallin, and glucose-regulated protein 58kDa). Conversely, enzymes of glycolysis were overexpressed in the lighter group. Such samples were also characterized by higher levels of glutathione S-transferase omega), which can activate the RyR calcium channels, and higher levels of cyclophilin D. This protein pattern is likely to have severe implications on postmortem metabolism, namely, acceleration of ATP depletion and pH fall and subsequent enhanced protein denaturation, well-known to induce discoloration.
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Dates and versions

hal-02665535 , version 1 (31-05-2020)

Identifiers

  • HAL Id : hal-02665535 , version 1
  • PRODINRA : 33505
  • WOS : 000236628500040

Cite

Thierry Sayd, Martine Morzel, Christophe C. Chambon, Michel Franck, Philippe Figwer, et al.. Proteome analysis of the sarcoplasmic fraction of pig semimembranosus muscle: implication on meat color development. Journal of Agricultural and Food Chemistry, 2006, 54 (7), pp.2732-2737. ⟨hal-02665535⟩
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