Viable but non-culturable Listeria monocytogenes on parsley leaves and absence of recovery to a culturable state - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Journal of Applied Microbiology Year : 2007

Viable but non-culturable Listeria monocytogenes on parsley leaves and absence of recovery to a culturable state

Abstract

To investigate the presence of viable but non-culturable Listeria monocytogenes during survival on parsley leaves under low relative humidity (RH) and to evaluate the ability of L. monocytogenes to recover from VBNC to culturable state under satured humidity. Methods and Results: Under low RH (47–69%) on parsley leaves, the initial number of L. monocytogenes populations counted on non selective media (109 L. monocytogenes per leaf on TSA) was reduced by 6 log10 scales in 15 days, whereas number of viable L. monocytogenes counted under the microscope was reduced by 3–4 log10 scales, indicating the presence of VBNC cells. This was demonstrated on three L. monocytogenes strains (EGDe, Bug 1995 and LmP60). Changing from low to 100% RH permitted an increase of the culturable counts of L. monocytogenes and this growth was observed only when residual culturable cells were present. Moreover, VBNC L. monocytogenes inoculated on parsley leaves did not become culturable after incubation under 100% RH. Conclusions: Dry conditions induced VBNC L. monocytogenes on parsley leaves but these VBNC were likely unable to recover culturability after transfer to satured humidity.

Dates and versions

hal-02665988 , version 1 (31-05-2020)

Identifiers

Cite

Nicolas Dreux, Guy Albagnac, Michel M. Federighi, Frederic F. Carlin, Cindy E. Morris, et al.. Viable but non-culturable Listeria monocytogenes on parsley leaves and absence of recovery to a culturable state. Journal of Applied Microbiology, 2007, 103 (4), pp.1272-1281. ⟨10.1111/j.1365-2672.2007.03351.x⟩. ⟨hal-02665988⟩
16 View
0 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More