Insulin and amino acid availability regulate atrogin-1 in avian QT6 cells - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue Biochemical and Biophysical Research Communications Année : 2007

Insulin and amino acid availability regulate atrogin-1 in avian QT6 cells

Résumé

New evidence has demonstrated that the expression of major genes, termed atrogenes, controls the ubiquitin– roteasome proteolytic pathway. The present work aimed to study the impact of insulin and amino acids on the expression of one of these atrogenes, the E3 ubiquitin ligase Muscle Atrophy F box (MAFbx, also called atrogin-1), in quail muscle (QT6) fibroblasts. First, we characterized atrogin- 1 in QT6 cells and demonstrated the insulin sensitivity of these cells. Second, we showed that insulin reduced atrogin-1 mRNA via the phosphatidylinositol-30kinase (PI3K)/protein kinase B (PKB or AKT)/target of rapamycin (TOR) pathway. Atrogin-1 expression also depended on the availability of an individual amino acid, i.e., methionine. Moreover, the amino acid-induced reduction of atrogin-1 was inhibited by rapamycin, indicating the involvement of the TOR pathway in such regulation. In conclusion, expression of the ubiquitin ligase atrogin-1 is regulated by both insulin and amino acids through the TOR pathway
Fichier non déposé

Dates et versions

hal-02666169 , version 1 (31-05-2020)

Identifiants

  • HAL Id : hal-02666169 , version 1
  • PRODINRA : 13705
  • WOS : 000246028800030

Citer

Sophie Tesseraud, Sonia Metayer-Coustard, Sourour Boussaid, Sabine Crochet, Estelle Audouin, et al.. Insulin and amino acid availability regulate atrogin-1 in avian QT6 cells. Biochemical and Biophysical Research Communications, 2007, 357, pp.181-186. ⟨hal-02666169⟩
5 Consultations
0 Téléchargements

Partager

Gmail Facebook X LinkedIn More