Insulin and amino acid availability regulate atrogin-1 in avian QT6 cells - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Biochemical and Biophysical Research Communications Year : 2007

Insulin and amino acid availability regulate atrogin-1 in avian QT6 cells

Abstract

New evidence has demonstrated that the expression of major genes, termed atrogenes, controls the ubiquitin– roteasome proteolytic pathway. The present work aimed to study the impact of insulin and amino acids on the expression of one of these atrogenes, the E3 ubiquitin ligase Muscle Atrophy F box (MAFbx, also called atrogin-1), in quail muscle (QT6) fibroblasts. First, we characterized atrogin- 1 in QT6 cells and demonstrated the insulin sensitivity of these cells. Second, we showed that insulin reduced atrogin-1 mRNA via the phosphatidylinositol-30kinase (PI3K)/protein kinase B (PKB or AKT)/target of rapamycin (TOR) pathway. Atrogin-1 expression also depended on the availability of an individual amino acid, i.e., methionine. Moreover, the amino acid-induced reduction of atrogin-1 was inhibited by rapamycin, indicating the involvement of the TOR pathway in such regulation. In conclusion, expression of the ubiquitin ligase atrogin-1 is regulated by both insulin and amino acids through the TOR pathway
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Dates and versions

hal-02666169 , version 1 (31-05-2020)

Identifiers

  • HAL Id : hal-02666169 , version 1
  • PRODINRA : 13705
  • WOS : 000246028800030

Cite

Sophie Tesseraud, Sonia Metayer-Coustard, Sourour Boussaid, Sabine Crochet, Estelle Audouin, et al.. Insulin and amino acid availability regulate atrogin-1 in avian QT6 cells. Biochemical and Biophysical Research Communications, 2007, 357, pp.181-186. ⟨hal-02666169⟩
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