Skip to Main content Skip to Navigation
Journal articles

Isolation and characterization of polymorphic nuclear microsatellite loci in Taxus baccata L.

Abstract : Seven polymorphic nuclear microsatellite markers for Taxus baccata L. (English yew) were developed using an enriched-library method. An additional polymorphic SSR was obtained by testing eight primer pairs from the congeneric species Taxus sumatrana. Mendelian inheritance for the seven Taxus baccata SSRs was proved by genotyping 17 individuals and 124 megagametophytes (conifer seed haploid tissue). A total of 96 individuals from 5 different populations (10–26 samples per population) were used to estimate genetic diversity parameters. High levels of genetic diversity, with values ranging from 0.533 to 0.929 (6–28 alleles per SSR) were found. No linkage disequilibrium between pairs of loci was detected. All loci but one showed significant departures from Hardy–Weinberg equilibrium. Excess of homozygosity was probably due to high inbreeding in English yew populations, an outcome of low effective population size and/or fragmented distribution. Highly polymorphic SSRs will be used to conduct population genetic studies at different geographical scales and to monitor gene flow.
Document type :
Journal articles
Complete list of metadata

https://hal.inrae.fr/hal-02666226
Contributor : Migration Prodinra Connect in order to contact the contributor
Submitted on : Sunday, May 31, 2020 - 8:17:01 AM
Last modification on : Friday, October 8, 2021 - 11:54:10 AM

Links full text

Identifiers

Collections

Citation

Marta Dubreuil, Federico Sebastiani, Maria Mayol, Santiago C. González-Martínez, Miquel Riba, et al.. Isolation and characterization of polymorphic nuclear microsatellite loci in Taxus baccata L.. Conservation Genetics, Springer Verlag, 2008, 9 (6), pp.1665-1668. ⟨10.1007/s10592-008-9515-3⟩. ⟨hal-02666226⟩

Share

Metrics

Record views

21