Dual effect of organic acids as a function of external pH in Oenococcus oeni - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Archives of Microbiology Year : 2007

Dual effect of organic acids as a function of external pH in Oenococcus oeni

Yoann Augagneur
  • Function : Author
Jean-Francois Ritt
  • Function : Author
Fabienne Remize
Dominique D. Garmyn
  • Function : Author
  • PersonId : 1203170
Jean Guzzo
  • Function : Author

Abstract

In this study we analyzed under various pH conditions including low pH, the effects of l-malic acid and citric acid, combined or not, on the growth, the proton motive force components and the transcription level of selected genes of the heterolactic bacterium Oenococcus oeni. It is shown here that l-malate enhanced the growth yield at pH equal or below 4.5 while the presence of citrate in media led to a complete and unexpected inhibition of the growth at pH 3.2. Nevertheless, whatever the growth conditions, both l-malate and citrate participated in the enhancement of the transmembrane pH gradient, whereas the membrane potential decreased with the pH. These results suggested that it was not citrate that was directly responsible for the inhibition observed in cultures done at low pH, but probably its end products. This was confirmed since, in media containing l-malate, the addition of acetate substantially impaired the growth rate of the bacterium and slightly the membrane potential and pH gradient. Finally, study of the expression of genes involved in the metabolism of organic acids showed that at pH 4.5 and 3.2 the presence of l-malate led to an increased amount of mRNA of mleP encoding a malate transporter.

Dates and versions

hal-02666371 , version 1 (31-05-2020)

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Yoann Augagneur, Jean-Francois Ritt, Daniel M. Linares, Fabienne Remize, Raphaëlle Tourdot-Maréchal, et al.. Dual effect of organic acids as a function of external pH in Oenococcus oeni. Archives of Microbiology, 2007, 188 (2), pp.147-157. ⟨10.1007/s00203-007-0230-0⟩. ⟨hal-02666371⟩
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