Validation of the performance of a GMO multiplex screening assay based on microarray detection
Serge Leimanis
(1)
,
Sandrine Hamels
(1)
,
Florance Nazé
(2)
,
Guillaume Mbongolo Mbella
(2)
,
Myriam Sneyers
(2)
,
Rupert Hochegger
(3)
,
Hermann Broll
(4)
,
Lilian Roth
(4)
,
Klara Dallmann
(5)
,
Adrienn Micsinai
(5)
,
Jose Luis La Paz
(6)
,
Maria Pla
(6)
,
Claudia Brünen-Nieweler
(7)
,
Nina Papazova
(8)
,
Isabel Tavernier
(8)
,
Norbert Hess
(9)
,
Britta Kirschneit
(9)
,
Yves Bertheau
(10)
,
Colette C. Audeon
(11)
,
Valerie Laval
(11)
,
Ulrich Busch
(12)
,
Sven Pecoraro
(12)
,
Katrin Neumann
(13)
,
Sibylle Rösel
(13)
,
Jeroen van Dijk
(14)
,
Esther Kok
(14)
,
Gianni Bellocchi
(15)
,
Nicoletta Foti
(16)
,
Marco Mazzara
(16)
,
William Moens
(16)
,
Jose Remacle
(1)
,
Guy van den Eede
(16)
1
Eppendorf Array Technologies
2 Institut Scientifique de la Santé Publique - Louis Pasteur
3 Austrian Agency for Health and Food Safety
4 BfR - Bundesinstitut für Risikobewertung - Federal Institute for Risk Assessment
5 Biomi Ltd.
6 CSIC - Consejo Superior de Investigaciones Cientificas [España] = Spanish National Research Council [Spain]
7 Chemische Landes- und Staatliche Veterinäruntersuchungsamt
8 ILVO - Research Institute for Agricultural, Fisheries and Food
9 Institute for Hygiene and Environment
10 Services déconcentrés d'appui à la recherche Ile-de-France-Versailles-Grignon
11 BIOGER - BIOlogie et GEstion des Risques en agriculture
12 Bavarian Health and Food Safety Authority [Oberschleißheim, Germany]
13 Lifeprint GmbH
14 WUR - Wageningen University and Research Centre
15 European Commission
16 Institute for Health and Consumer Protection of the JRC
2 Institut Scientifique de la Santé Publique - Louis Pasteur
3 Austrian Agency for Health and Food Safety
4 BfR - Bundesinstitut für Risikobewertung - Federal Institute for Risk Assessment
5 Biomi Ltd.
6 CSIC - Consejo Superior de Investigaciones Cientificas [España] = Spanish National Research Council [Spain]
7 Chemische Landes- und Staatliche Veterinäruntersuchungsamt
8 ILVO - Research Institute for Agricultural, Fisheries and Food
9 Institute for Hygiene and Environment
10 Services déconcentrés d'appui à la recherche Ile-de-France-Versailles-Grignon
11 BIOGER - BIOlogie et GEstion des Risques en agriculture
12 Bavarian Health and Food Safety Authority [Oberschleißheim, Germany]
13 Lifeprint GmbH
14 WUR - Wageningen University and Research Centre
15 European Commission
16 Institute for Health and Consumer Protection of the JRC
Yves Bertheau
- Fonction : Auteur
- PersonId : 529
- IdHAL : yves-bertheau
- ORCID : 0000-0002-9641-7104
- IdRef : 16043307X
Valerie Laval
- Fonction : Auteur
- PersonId : 753274
- IdHAL : valerie-laval
- ORCID : 0000-0002-3667-3878
Gianni Bellocchi
- Fonction : Auteur
- PersonId : 744818
- IdHAL : gianni-bellocchi
- ORCID : 0000-0003-2712-7979
- IdRef : 176945385
Résumé
A new screening method for the detection and identification of GMO, based on the use of multiplex PCR followed by microarray, has been developed and is presented. The technology is based on the identification of quite ubiquitous GMO genetic target elements first amplified by PCR, followed by direct hybridisation of the amplicons on a predefined microarray (DualChip® GMO, Eppendorf, Germany). The validation was performed within the framework of a European project (Co-Extra, contract no 007158) and in collaboration with 12 laboratories specialised in GMO detection. The present study reports the strategy and the results of an ISO complying validation of the method carried out through an inter-laboratory study. Sets of blind samples were provided consisting of DNA reference materials covering all the elements detectable by specific probes present on the array. The GMO concentrations varied from 1% down to 0.045%. In addition, a mixture of two GMO events (0.1% RRS diluted in 100% TOPAS19/2) was incorporated in the study to test the robustness of the assay in extreme conditions. Data were processed according to ISO 5725 standard. The method was evaluated with predefined performance criteria with respect to the EC CRL method acceptance criteria. The overall method performance met the acceptance criteria; in particular, the results showed that the method is suitable for the detection of the different target elements at 0.1% concentration of GMO with a 95% accuracy rate. This collaborative trial showed that the method can be considered as fit for the purpose of screening with respect to its intra- and inter-laboratory accuracy. The results demonstrated the validity of combining multiplex PCR with array detection as provided by the DualChip® GMO (Eppendorf, Germany) for the screening of GMO. The results showed that the technology is robust, practical and suitable as a screening tool.