Kinetic of production and mode of action of the Lactobacillus paracasei subsp. paracasei anti-listerial bacteriocin, an Algerian isolate - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles LWT - Food Science and Technology Year : 2008

Kinetic of production and mode of action of the Lactobacillus paracasei subsp. paracasei anti-listerial bacteriocin, an Algerian isolate

Abstract

Lactobacillus paracasei subsp. paracasei was isolated in our laboratory from breast-fed newborn faeces and identified phenotypically and genotypically. The strain was able to produce a bacteriocin-like substance active towards listerial strains (Listenia innocua CLIP 74915 and Listeria monocytogenes EGDe). The maximum production of the substance by producing strain was detected in the late logarithmic growth phase (14 h in MRS and 18 h in BHI broths). It displayed bactericidal mode of action with leakage of cellular content (K+ and ATP) leading to cell lysis as secondary effect. A reduction of about 5 log with 35.7 +/- 0.2% of cell lysis and of about 4 log with 82.0 +/- 0.3% of cell lysis were observed, respectively, in a phosphate buffer and BHI suspensions. This was further demonstrated by electron microscopy that showed severe modifications in the cell morphology with a concomitant lysis. (C) 2008 Swiss Society of Food Science and Technology.

Dates and versions

hal-02667696 , version 1 (31-05-2020)

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Farida Bendali, Brigitte B. Gaillard-Martinie, Michel Hébraud, Djamila Sadoun. Kinetic of production and mode of action of the Lactobacillus paracasei subsp. paracasei anti-listerial bacteriocin, an Algerian isolate. LWT - Food Science and Technology, 2008, 41 (10), pp.1784-1792. ⟨10.1016/j.lwt.2008.02.010⟩. ⟨hal-02667696⟩

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