Skip to Main content Skip to Navigation
Journal articles

Phylogenetic analysis, genomic organization, and expression analysis of multi-copper oxidases in the ectomycorrhizal basidiomycete Laccaria bicolor

Abstract : In forest soils, ectomycorrhizal and saprotrophic Agaricales differ in their strategies for carbon acquisition, but share common gene families encoding multi-copper oxidases (MCOs). These enzymes are involved in the oxidation of a variety of soil organic compounds. The MCO gene family of the ectomycorrhizal fungus Laccaria bicolor is composed of 11 genes divided into two distinct subfamilies corresponding to laccases (lcc) sensu stricto (lcc1 to lcc9), sharing a high sequence homology with the coprophilic Coprinopsis cinerea laccase genes, and to ferroxidases (lcc10 and lcc11) that are not present in C. cinerea. The fet3-like ferroxidase genes lcc10 and lcc11 in L. bicolor are each arranged in a mirrored tandem orientation with an ftr gene coding for an iron permease. Unlike C. cinerea, L. bicolor has no sid1/sidA gene for siderophore biosynthesis. Transcript profiling using whole-genome expression arrays and quantitative reverse transcriptase–polymerase chain reaction (qRT-PCR) revealed that some transcripts were very abundant in ectomycorrhizas (lcc3 and lcc8), in fruiting bodies (lcc7) or in the free-living mycelium grown on agar medium (lcc9 and lcc10), suggesting a specific function of these MCOs. The amino acid composition of the MCO substrate binding sites suggests that L. bicolor MCOs interact with substrates different from those of saprotrophic fungi.
Document type :
Journal articles
Complete list of metadata

https://hal.inrae.fr/hal-02667828
Contributor : Migration Prodinra <>
Submitted on : Sunday, May 31, 2020 - 11:31:16 AM
Last modification on : Friday, February 5, 2021 - 3:39:49 AM

Links full text

Identifiers

Collections

Citation

Pierre-Emmanuel Courty, P.J. Hoegger, S. Kilaru, Annegret Kohler, Marc Buee, et al.. Phylogenetic analysis, genomic organization, and expression analysis of multi-copper oxidases in the ectomycorrhizal basidiomycete Laccaria bicolor. New Phytologist, Wiley, 2009, 182 (3), pp.736-750. ⟨10.1111/j.1469-8137.2009.02774.x⟩. ⟨hal-02667828⟩

Share

Metrics

Record views

14