Skip to Main content Skip to Navigation
Journal articles

Chemical sensing of DNT by engineered olfactory yeast strain.

Abstract : With the increasing threat of environmental toxicants including biological and chemical warfare agents, fabricating innovative biomimetic systems to detect these harmful agents is critically important. With the broad objective of developing such a biosensor, here we report the construction of a Saccharomyces cerevisiae strain containing the primary components of the mammalian olfactory signaling pathway. In this engineered yeast strain, WIF-1alpha, olfactory receptor signaling is coupled to green fluorescent protein expression. Using this 'olfactory yeast', we screened for olfactory receptors that could report the presence of the odorant 2,4-dinitrotoluene, an explosive residue mimic. With this approach, we have identified the novel rat olfactory receptor Olfr226, which is closely related to the mouse olfactory receptors Olfr2 and MOR226-1, as a 2,4-dinitrotoluene-responsive receptor.
Document type :
Journal articles
Complete list of metadata

https://hal.inrae.fr/hal-02667846
Contributor : Migration Prodinra <>
Submitted on : Sunday, May 31, 2020 - 11:33:08 AM
Last modification on : Wednesday, March 10, 2021 - 3:54:03 PM

Identifiers

Collections

Citation

Venkat Radhika, Tassula Proikas-Cezanne, Muralidharan Jayaraman, Djamila Onesime, Ji Hee Ha, et al.. Chemical sensing of DNT by engineered olfactory yeast strain.. Nature Chemical Biology, Nature Publishing Group, 2007, 3 (6), pp.325-30. ⟨10.1038/nchembio882⟩. ⟨hal-02667846⟩

Share

Metrics

Record views

16