Crystal structure of the Murray Valley encephalitis virus NS5 methyltransferase domain in complex with cap analogues
Résumé
We have determined the high resolution crystal structure of the m ethylt ransfe rase domain of the NS5 polypeptide from the Murray Valley encephalitis virus. This domain is unusual in having both the N7 and 2'-O methyltransferase activity required for Cap 1 synthesis. We have also determined structures for complexes of this domain with nucleoticles and cap analogues providing information on cap binding, based on which we suggest a model of how the sequential methylation of the N7 and 2'-O groups of the cap may be coordinated.