Insertional mutagenesis reveals genes involved in <em>Bacillus cereus</em> ATCC 14579 growth at low temperature - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles FEMS Microbiology Letters Year : 2010

Insertional mutagenesis reveals genes involved in Bacillus cereus ATCC 14579 growth at low temperature

Abstract

Transposon mutagenesis of Bacillus cereus ATCC 14579 yielded cold-sensitive mutants. Mutants of genes encoding enzymes of the central metabolism were affected by cold, but also by other stresses, such as pH or salt, whereas a mutant with transposon insertion in the promoter region of BC0259 gene, encoding a putative DEAD-box RNA helicase displaying homology with Escherichia coli CsdA and Bacillus subtilis CshA RNA helicases, was only cold-sensitive. Expression of the BC0259 gene at 10 °C is reduced in the mutant. Analysis of the 5' untranslated region revealed the transcriptional start and putative cold shock-responsive elements. The role of this RNA helicase in the cold-adaptive response of B. cereus is discussed

Dates and versions

hal-02668630 , version 1 (31-05-2020)

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Veronique V. Broussolle, Franck Pandiani, Nabila Haddad, Caroline Michaud, Frederic F. Carlin, et al.. Insertional mutagenesis reveals genes involved in Bacillus cereus ATCC 14579 growth at low temperature. FEMS Microbiology Letters, 2010, 306 (2), pp.177-183. ⟨10.1111/j.1574-6968.2010.01953.x⟩. ⟨hal-02668630⟩
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