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Generation of polyclonal antibodies against multiple proteins in a single rabbit and subsequent isolation of the specific immunoglobulins as a tool for proteomics research

Abstract : A single rabbit has been immunized with a mixture of six different proteins: green fluorescent protein, ferredoxin-NADP oxidoreductase, NADPH-thioredoxin reductase, thioredoxin f , fructose bisphosphatase and cyclophilin. Individual IgGs were re-purified from the serum through specific interaction on small columns generated by grafting the antigen on a CNBr-Sepharose matrix. The specificity of the isolated IgG was checked by Western-blot analysis. Purified and specific IgG fractions were obtained with all antigens except thioredoxin f .
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https://hal.inrae.fr/hal-02671937
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Submitted on : Sunday, May 31, 2020 - 3:56:53 PM
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Jean-Pierre Jacquot, Nicolas Rouhier, Nicolas Navrot, Jean-Pierre Decottignies. Generation of polyclonal antibodies against multiple proteins in a single rabbit and subsequent isolation of the specific immunoglobulins as a tool for proteomics research. Biotechnology and Applied Biochemistry, Wiley, 2004, 40 (2), pp.181-185. ⟨10.1042/BA20030196⟩. ⟨hal-02671937⟩

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