Interleukin-I (IL-1) system gene expression in granulosa cells : kinetics during terminal preovulatory follicle maturation in the mare - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue Reproductive Biology and Endocrinology Année : 2003

Interleukin-I (IL-1) system gene expression in granulosa cells : kinetics during terminal preovulatory follicle maturation in the mare

Résumé

BACKGROUND: A growing body of evidences suggests that the ovary is a site of inflammatory reactions, and thus, ovarian cells could represent sources and targets of the interleukin-1 (IL-1) system. The purpose of this study was to examine the IL-1 system gene expressions in equine granulosa cells, and to study the IL-1beta content in follicular fluid during the follicle maturation. For this purpose, granulosa cells and follicular fluids were collected from the largest follicle at the early dominance stage (diameter 24 +/- 3 mm) or during the preovulatory maturation phase, at T0 h, T6 h, T12 h, T24 h and T34 h after induction of ovulation. Cells were analysed by RT-PCR and follicular fluids were studied by gel electrophoresis and immunoblotting. RESULTS: We demonstrated that interleukin-1beta (IL-1beta), interleukin-1 receptor 2 (IL-1R2) and interleukin-1 receptor antagonist (IL-1RA) genes are expressed in equine granulosa cells. We observed that the IL-1beta and IL-1RA mRNA content changed in granulosa cells during the terminal follicular maturation whereas IL-1R2 mRNA did not vary. In follicular fluid, IL-1beta content fluctuated few hours after induction of ovulation. CONCLUSIONS: The expression of IL-1beta gene in granulosa cells and the follicular fluid IL-1beta content seem to be regulated by gonadotropins suggesting that IL-1beta could be an intermediate paracrine factor involved in ovulation.

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Autre [q-bio.OT]
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Dates et versions

hal-02672165 , version 1 (31-05-2020)

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Alain Martoriati, Nadine Gérard. Interleukin-I (IL-1) system gene expression in granulosa cells : kinetics during terminal preovulatory follicle maturation in the mare. Reproductive Biology and Endocrinology, 2003, 1, pp.1-10. ⟨10.1186/1477-7827-1-42⟩. ⟨hal-02672165⟩
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