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Characterization of a Lactococcus lactis strain that secretes a major epitope of bovine β-lactoglobulin and evaluation of its immunogenicity in mice.-

Abstract : Bovine beta-lactoglobulin (Blg) is one of the major cow's milk allergens. Peptide 41-60 of Blg (Blg41-60) was described as a murine T-cell determinant and a murine, rat, and human immunoglobulin E (IgE) epitope. The aim of this study was the expression of Blg41-60 as a fusion protein in the food-grade bacterium Lactococcus lactis and the characterization of its immunogenicity in mice. We constructed a recombinant strain of L. lactis capable of inducible production and secretion of Blg41-60::Nuc, a fusion protein between Blg41-60 and the mature part of the staphylococcal nuclease (Nuc). The highest production yield of Blg41-60::Nuc (32.5 mg/liter) was reached 4 h after induction. At this time, up to 75% of Blg41-60::Nuc was secreted. When monoclonal antibodies specific for Blg41-60 were used, purified Blg41-60::Nuc and synthetic Blg41-60 exhibited very similar immunoreactivities. Subcutaneous coadministration of purified Blg41-60::Nuc and killed nonrecombinant L. lactis resulted in the induction of specific anti-Blg41-60 IgG2a and IgG1. The IgG1/IgG2a ratio and the lack of specific IgE suggest a Th1-type immune response, i.e., a nonallergic response. Similar administrations of the killed Blg41-60::Nuc-producing L. lactis strain did not elicit a specific immune response, whereas a transitory mucosal IgA-specific immune response was induced in mice after oral administration of the live Blg41-60::Nuc-producing L. lactis strain.
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https://hal.inrae.fr/hal-02677536
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Submitted on : Sunday, May 31, 2020 - 7:30:41 PM
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Jean-Marc Chatel, Sebastien Nouaille, Karine Adel-Patient, Yves Le Loir, Herman Boe, et al.. Characterization of a Lactococcus lactis strain that secretes a major epitope of bovine β-lactoglobulin and evaluation of its immunogenicity in mice.-. Applied and Environmental Microbiology, American Society for Microbiology, 2003, 69 (11), pp.6620-6627. ⟨10.1128/AEM.69.11.6620–6627.⟩. ⟨hal-02677536⟩

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