Oligomeric compounds formed from 2,5-xylidine (2,5-dimethylaniline) are potent enhancers of laccase production in Trametes versicolor ATCC 32745 - Archive ouverte HAL Access content directly
Journal Articles Applied Microbiology and Biotechnology Year : 2005

Oligomeric compounds formed from 2,5-xylidine (2,5-dimethylaniline) are potent enhancers of laccase production in Trametes versicolor ATCC 32745

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Abstract

Numerous chemicals, including the xenobiotic 2,5-xylidine, are known to induce laccase production in fungi. The present study was conducted to determine whether the metabolites formed from 2,5-xylidine by fungi could enhance laccase activity. We used purified laccases to transform the chemical and then we separated the metabolites, identified their chemical structure and assayed their effect on enzyme activity in liquid cultures of Trametes. versicolor. We identified 13 oligomers formed from 2,5-xylidine. (4E)-4-(2,5-dimethylphenylimino)-2,5-dimethylcyclohexa-2,5-dienone at 1.25×10−5 M was an efficient inducer, resulting in a nine-fold increase of laccase activity after 3 days of culture. Easily synthesized in one step (67% yield), this compound could be used in fungal bioreactors to obtain a great amount of laccases for biochemical or biotechnological purposes, with a low amount of inducer.
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hal-02678506 , version 1 (20-01-2023)

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Attribution - ShareAlike - CC BY 4.0

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Albert Kollmann, Francois Boyer, Paul Henri Ducrot, Lucien Kerhoas, Claude Jolivalt, et al.. Oligomeric compounds formed from 2,5-xylidine (2,5-dimethylaniline) are potent enhancers of laccase production in Trametes versicolor ATCC 32745. Applied Microbiology and Biotechnology, 2005, 68 (2), pp.251-258. ⟨10.1007/s00253-004-1860-7⟩. ⟨hal-02678506⟩

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