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CD208/dendritic cell-lysosomal associated membrane protein is a marker of normal and transformed type II pneumocytes

Abstract : Dendritic cell-lysosomal associated membrane protein (DC-LAMP)/CD208, a member of the lysosomal associated membrane protein (LAMP) family, is specifically expressed by human DCs on activation. However, its mouse counterpart could not be detected in mature DCs. The present study demonstrates that DC-LAMP is constitutively expressed by mouse, sheep, and human type H pneumocytes. Confocal and immunoelectron microscopy showed that mouse DC-LAMP protein co-localizes with Ibm180, a specific marker for the limiting membrane of lamellar bodies that contain surfactant protein B, as well as with intracellular MHC class H molecules that accumulate in the same organelles. Expression of DC-LAMP was also occasionally detected at the cell surface of type H pneumocytes. Interestingly, human bronchioloalveolar carcinoma tumor cells, which correspond to transformed type II pneumocytes, express DC-LAMP. Similar observations were made in the Jaagsiekte sheep retrovirus-associated ovine pulmonary adenocarcinoma, a model of human bronchioloalveolar carcinoma. This study establishes that DC-LAMP is constitutively expressed in normal type H pneumocytes. Furthermore, DC-LAMP appears to be a marker of transformed type 11 pneumocytes as well, an observation that may help the study and the classification of human lung adenocarcinomas.
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https://hal.inrae.fr/hal-02679874
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Submitted on : Sunday, May 31, 2020 - 9:48:20 PM
Last modification on : Friday, March 5, 2021 - 3:29:48 AM

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Bruno Salaun, Blandine de Saint-Vis, Nathalie Pacheco, Yves Pacheco, Arnaud Riesler, et al.. CD208/dendritic cell-lysosomal associated membrane protein is a marker of normal and transformed type II pneumocytes. American Journal of Pathology, American Society for Investigative Pathology, 2004, 164 (3), pp.861-871. ⟨10.1016/S0002-9440(10)63174-4⟩. ⟨hal-02679874⟩

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