Interference of soil contaminants with laccase activity during the transformation of complex mixtures of polycyclic aromatic hydrocarbons in liquid media - Archive ouverte HAL Access content directly
Journal Articles Polycyclic Aromatic Compounds Year : 2002

Interference of soil contaminants with laccase activity during the transformation of complex mixtures of polycyclic aromatic hydrocarbons in liquid media

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Abstract

The biotransformation of 16 polycyclic aromatic hydrocarbons (PAHs) in mixture was investigated in reactors in the presence of purified laccases of the fungus Pycnoporus cinnabarinus, ABTS as a redox mediator, 25% acetonitrile, and Tween 20. Several hydrocarbons from a synthetic mixture, such as anthracene and benzo[ a ]pyrene, were converted up to 80% into quinones, whereas others also belonging to three- and five-ring chemicals were less transformed. Chrysene and benzo[ k ]fluoranthene were not oxidized by the laccase mediator system. Moreover, hydrocarbons extracted from an industrial soil were all recalcitrant to enzymatic attack. This lack of reactivity of the laccases toward the hydrocarbons could be due to the presence of interfering compounds coextracted from the soil, such as metals.
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hal-02681745 , version 1 (18-01-2023)

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Attribution - ShareAlike - CC BY 4.0

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Christian Mougin, Claude Jolivalt, C. Malosse, Véronique Chaplain, J.C. Sigoillot, et al.. Interference of soil contaminants with laccase activity during the transformation of complex mixtures of polycyclic aromatic hydrocarbons in liquid media. Polycyclic Aromatic Compounds, 2002, 22 (3-4), pp.673-688. ⟨10.1080/10406630213591⟩. ⟨hal-02681745⟩
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