Sequencing of Aspergillus nidulans and comparative analysis with A-fumigatus and A-oryzae
James Galagan
(1)
,
Sarah Calvo
(1)
,
Christina Cuomo
(1)
,
Ma Li-Jun
(1)
,
Jennifer Wortman
(2)
,
Serafim Batzoglou
(3)
,
Lee Su-In
(3)
,
Meray Bastürkmen
(4)
,
Christina Spevak
(4)
,
John Clutterbuck
(5)
,
Vladimir Kapitonov
(6)
,
Jerzy Jurka
(6)
,
Claudio Scazzocchio
(7)
,
Mark Farman
(8)
,
Jonathan Butler
(1)
,
Seth Purcell
(1)
,
Steve Harris
(9)
,
Gerhard Braus
(10)
,
Oliver Draht
(10)
,
Silke Busch
(10)
,
Christophe D'Enfert
(11)
,
Christiane Bouchier
(12)
,
Gustavo Goldman
(13)
,
Deborah Bell-Pedersen
(14)
,
Sam Griffiths-Jones
(15)
,
John Doonan
(16)
,
Jaehyuk Yu
(17)
,
Kay Vieken
(18)
,
Arnab Pain
(15)
,
Michael Freitag
(19)
,
Eric Selker
(19)
,
David Archer
(20)
,
Miguel Peñalva
(21)
,
Berl Oakley
(22)
,
Michelle Momany
(23)
,
Toshihiro Tanaka
(24)
,
Toshitaka Kumagai
(25)
,
Kiyoshi Asai
(25)
,
Masayuki Machida
(25)
,
William Nierman
(2)
,
David Denning
(26)
,
Mark Caddik
(27)
,
Michael Hynes
(28)
,
Mathieu Paoletti
(20)
,
Reinhard Fischer
(18)
,
Bruce Miller
(29)
,
Paul Dyer
(20)
,
Matthew Sachs
(4)
,
Stephen Osmani
(30)
,
Bruce Birren
(1)
1
MIT -
Massachusetts Institute of Technology
2 TIGR - The Institute for Genomic Research
3 Stanford University
4 Oregon Health & Science University
5 University of Glasgow
6 Genetic Information Research Institute
7 IGM - Institut de génétique et microbiologie [Orsay]
8 Department of Plant Pathology
9 University of Nebraska System
10 Georg-August-University = Georg-August-Universität Göttingen
11 BPF - Biologie et Pathogénicité fongiques
12 IP - Institut Pasteur [Paris]
13 USP - Universidade de São Paulo = University of São Paulo
14 Department of Biology
15 The Wellcome Trust Sanger Institute [Cambridge]
16 John Innes Centre [Norwich]
17 University of Wisconsin-Madison
18 Max Planck Institute for Terrestrial Microbiology
19 Institute of Molecular Biology
20 UON - University of Nottingham, UK
21 Centro de Investigaciones Biologicas
22 Department of Molecular Genetics
23 Department of Plant Biology
24 NIT - National Institute of Technology [Patna]
25 AIST - Advanced Industrial Science and technology
26 University of Manchester [Manchester]
27 University of Liverpool
28 Department of Genetics
29 University of Idaho [Moscow, USA]
30 OSU - Ohio State University [Columbus]
2 TIGR - The Institute for Genomic Research
3 Stanford University
4 Oregon Health & Science University
5 University of Glasgow
6 Genetic Information Research Institute
7 IGM - Institut de génétique et microbiologie [Orsay]
8 Department of Plant Pathology
9 University of Nebraska System
10 Georg-August-University = Georg-August-Universität Göttingen
11 BPF - Biologie et Pathogénicité fongiques
12 IP - Institut Pasteur [Paris]
13 USP - Universidade de São Paulo = University of São Paulo
14 Department of Biology
15 The Wellcome Trust Sanger Institute [Cambridge]
16 John Innes Centre [Norwich]
17 University of Wisconsin-Madison
18 Max Planck Institute for Terrestrial Microbiology
19 Institute of Molecular Biology
20 UON - University of Nottingham, UK
21 Centro de Investigaciones Biologicas
22 Department of Molecular Genetics
23 Department of Plant Biology
24 NIT - National Institute of Technology [Patna]
25 AIST - Advanced Industrial Science and technology
26 University of Manchester [Manchester]
27 University of Liverpool
28 Department of Genetics
29 University of Idaho [Moscow, USA]
30 OSU - Ohio State University [Columbus]
James Galagan
- Fonction : Auteur
- PersonId : 758169
- ORCID : 0000-0003-0542-3291
Christina Cuomo
- Fonction : Auteur
- PersonId : 774773
- ORCID : 0000-0002-5778-960X
Gerhard Braus
- Fonction : Auteur
- PersonId : 761743
- ORCID : 0000-0002-3117-5626
Christophe D'Enfert
- Fonction : Auteur
- PersonId : 801320
- ORCID : 0000-0002-6235-3886
- IdRef : 073388890
Christiane Bouchier
- Fonction : Auteur
- PersonId : 756591
- ORCID : 0000-0003-3549-1543
Gustavo Goldman
- Fonction : Auteur
- PersonId : 761744
- ORCID : 0000-0002-2986-350X
Arnab Pain
- Fonction : Auteur
- PersonId : 777756
- ORCID : 0000-0002-1755-2819
Mathieu Paoletti
- Fonction : Auteur
- PersonId : 742719
- IdHAL : mathieu-paoletti
Résumé
The aspergilli comprise a diverse group of filamentous fungi spanning over 200 million years of evolution. Here we report the genome sequence of the model organism Aspergillus nidulans, and a comparative study with Aspergillus fumigatus, a serious human pathogen, and Aspergillus oryzae, used in the production of sake, miso and soy sauce. Our analysis of genome structure provided a quantitative evaluation of forces driving long-term eukaryotic genome evolution. It also led to an experimentally validated model of mating-type locus evolution, suggesting the potential for sexual reproduction in A. fumigatus and A. oryzae. Our analysis of sequence conservation revealed over 5,000 non-coding regions actively conserved across all three species. Within these regions, we identified potential functional elements including a previously uncharacterized TPP riboswitch and motifs suggesting regulation in filamentous fungi by Puf family genes. We further obtained comparative and experimental evidence indicating widespread translational regulation by upstream open reading frames. These results enhance our understanding of these widely studied fungi as well as provide new insight into eukaryotic genome evolution and gene regulation.