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Essential Bacillus subtilis genes.

K Kobayashi 1 S. D. Ehrlich 2, * A Albertini 3 G Amati 3 K. K. Andersen 4 M. Arnaud 5 K. Asai 6 S. Ashikaga 7 Stephane Aymerich 8 Philippe Bessieres 9 F. Boland 10 S. C. Brignell 11 S. Bron 12 K. Bunai 13 Jérôme Chapuis 2 L. C. Christiansen 14 A. Danchin 15 Michel Debarbouille 5 Etienne Dervyn 2 E. Deuerling 16 K. Devine 4 S. K. Devine 4 O Dreesen 15 J. Errington 17 S. Fillinger 11 S. J. Foster 10 Y. Fujita 18 A. Galizzi 3 Rozenn Gardan 5 C Eschevins 12 T. Fukushima 19 K. Haga 20 C. R. Harwood 11 M. Hecker 21 D. Hosoya 22 M. F. Hullo 15 H. Kakeshita 13 D. Karamata 23 Y. Kasahara 1 F. Kawamura 7 K. Koga 7 P. Koski 24 R. Kuwana 25 D. Imamura 22 M. Ishimaru 22 S. Ishikawa 19 I. Ishio 18 Dominique Le Coq 8 A. Masson 26 C. Mauël 23 R. Meima 12 R. P. Mellado A. Moir 10 S. Moriya 1 E. Nagakawa 18 H. Nanamiya 7 S. Nakai 1 P. Nygaard 14 M. Ogura 27 T. Ohanan 16 M O'Reilly 4 M O'Rourke 10 Zoltan Pragai 11 H. M. Pooley 23 G. Rapoport 5 J. P. Rawlins 17 L A. Rivas C. Rivolta 23 A. Sadaie 20 Y. Sadaie 6 M. Sarvas 24 T. Sato 22 H H Saxild 14 E. Scanlan 4 W. Schumann 16 J. F. M. L. Seegers 26 J. Sekiguchi 19 A. Sekowska 15 S. J. Séror 26 M. Simon 28 P. Stragier 28 R. Studer 23 H. Takamatsu 25 T. Tanaka 27 M. Takeuchi 22 H. B. Thomaides 17 Valérie Vagner 2 J. M. van Dijl 12 K. Watabe 25 A. Wipat 11 H. Yamamoto 19 M. Yamamoto 18 Y. Yamamoto 18 K. Yamane 13 K. Yata 29 K Yoshida 18 H. Yoshikawa 20 U. Zuber 21 N. Ogasawara 1
Abstract : To estimate the minimal gene set required to sustain bacterial life in nutritious conditions, we carried out a systematic inactivation of Bacillus subtilis genes. Among approximately 4,100 genes of the organism, only 192 were shown to be indispensable by this or previous work. Another 79 genes were predicted to be essential. The vast majority of essential genes were categorized in relatively few domains of cell metabolism, with about half involved in information processing, one-fifth involved in the synthesis of cell envelope and the determination of cell shape and division, and one-tenth related to cell energetics. Only 4% of essential genes encode unknown functions. Most essential genes are present throughout a wide range of Bacteria, and almost 70% can also be found in Archaea and Eucarya. However, essential genes related to cell envelope, shape, division, and respiration tend to be lost from bacteria with small genomes. Unexpectedly, most genes involved in the Embden-Meyerhof-Parnas pathway are essential. Identification of unknown and unexpected essential genes opens research avenues to better understanding of processes that sustain bacterial life.
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K Kobayashi, S. D. Ehrlich, A Albertini, G Amati, K. K. Andersen, et al.. Essential Bacillus subtilis genes.. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2003, 100 (8), pp.4678-4683. ⟨10.1073/pnas.0730515100⟩. ⟨hal-02683417⟩

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