High resolution HPLC method with reverse phase C18 symetry TM column for the analysis of tetracyclines produced by Streptomyces aureofaciens immobilized in Ca-alginate gel
Résumé
To analyse the effect of immobilization of Streptomyces aureofaciens in Ca-alginate on tetracycline production, an HPLC technique for direct injection of the culture medium has been developed. The high resolution obtained with this method is achieved by using a Symmetry C18TM reverse phase HPLC column. The comparison of our results with those previously described by different authors, shows that highest performances are obtained without using relatively drastic conditions such as 0.2 M oxalic acid at pH 2. Thus, our method, which has shown a very good reproducibility for hundreds of injections, is also better for life-time column preservation.