Influence of rainbow trout thermal acclimation on sperm cryopreservation : relation to change in the lipid composition of the plasma membrane - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Aquaculture Year : 1996

Influence of rainbow trout thermal acclimation on sperm cryopreservation : relation to change in the lipid composition of the plasma membrane

Catherine Labbé
Gérard Maisse
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Abstract

The ability of rainbow trout (Oncorhynchus mykiss) spermatozoa to withstand cryopreservation was tested after the fish and sperm were exposed to various temperature conditions, In the first experiment, trout were acclimated at either 8 degrees C or 18 degrees C during gametogenesis, and transferred to 13 degrees C at the beginning of the spawning season, Following this 13 degrees C transfer, the fertilizing ability of frozen-thawed sperm from the fish reared at 18 degrees C was found to be twice as better (60-80% fertilization rate) than that of the fish reared at 8 degrees C (20-30% fertilization rate) up to 42 days after the transfer. A low membrane cholesterol/phospholipid ratio was strongly correlated (P < 0.002) with the better freezing resistance of the corresponding sperm. The relevance of this parameter in predicting sperm ability to survive cryopreservation is discussed, In another experiment, fish with the same thermal background during gametogenesis were transferred to 13 degrees C or to 5 degrees C before the beginning of the spawning season, The same fertilization rates after freeze-thawing were observed between the two groups (30-35%), Weekly or bi-weekly stripping frequencies did not alter the fertilization rates. Thus, no damage can be attributed to a long in vivo storage of sperm at a relatively high temperature, In an in vitro experiment, the storage temperature (0 degrees C to 25 degrees C) of sperm before freezing was shown to have little influence on the fertilizing results after thawing although membrane fluidity was higher in sperm stored at 24 degrees C compared with those stored at 4 degrees C. It is concluded from this study that the quality of sperm cryopreservation can be improved by appropriate broodstock handling. A high rearing temperature during gametogenesis followed by transfer to colder water improves sperm resistance during cryopreservation. The stripping frequency of the fish does not influence sperm quality at the temperature tested. Storing sperm in vitro below 5 degrees C is recommended, although higher temperatures are not markedly deleterious.

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Dates and versions

hal-02690954 , version 1 (01-06-2020)

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Catherine Labbé, Gérard Maisse. Influence of rainbow trout thermal acclimation on sperm cryopreservation : relation to change in the lipid composition of the plasma membrane. Aquaculture, 1996, 145, pp.281-294. ⟨10.1016/S0044-8486(96)01354-3⟩. ⟨hal-02690954⟩

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