Agrobacterium tumefaciens-mediated transformation of hybrid larch (Larix kaempferi x L. decidua) and transgenic plant regeneration - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Plant Cell Reports Year : 1997

Agrobacterium tumefaciens-mediated transformation of hybrid larch (Larix kaempferi x L. decidua) and transgenic plant regeneration

Abstract

A transformation procedure was developed for hybrid larch embryogenic tissue using Agrobacterium tumefaciens. The cocultivation procedure yielded one to two transformation events per 100 cocultivated masses. The addition of 100 mu M coniferyl alcohol increased the yield. This improved procedure was successfully applied to three other genotypes. After 3 months on selective medium, the transgenic tissue remained embryogenic, which allowed production of transgenic plants in the greenhouse. Stable integration of the transgene was confirmed by PCR and Southern hybridisation on transformed tissues and acclimatised plants.

Domains

Plants genetics

Dates and versions

hal-02695750 , version 1 (01-06-2020)

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Cite

Valérie Levée, Marie-Anne Lelu-Walter, Lise Jouanin, Daniel Cornu, Gilles Pilate. Agrobacterium tumefaciens-mediated transformation of hybrid larch (Larix kaempferi x L. decidua) and transgenic plant regeneration. Plant Cell Reports, 1997, 16 (10), pp.680-685. ⟨10.1007/s002990050301⟩. ⟨hal-02695750⟩

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