Skip to Main content Skip to Navigation
Journal articles

Exportation de protéines hétérologues chez Lactococcus lactis : développement d'outils moléculaires et applications

Abstract : Heterologous protein export in Lactococcus lactis : development of molecular tools and applications. The GRAS (for Generally Regarded As Safe) bacterium, Lactococcus lactis, is a good candidate to produce heterologous proteins of vaccinal, medical or technological interest. The export (secretion or cell wall anchoring) of such proteins would allow a direct contact between the protein and its target and therefore would be a better mode of production than intracellular production. To develop heterologous protein export systems in L. lactis, we used the staphylococcal nuclease. Three major results were obtained in L. lactis: (i) a synthetic propeptide has been identified which enhances secretion efficiency of heterologous proteins; (ii) a fusion protein has been designed which allows to target a protein of interest to three cellular localizations (secreted, anchored or intracellular); and (iii) new lactococcal export signals have been identified. These tools are being used to construct L. lactis strains able to export viral antigens and technological enzymes.
Document type :
Journal articles
Complete list of metadata

https://hal.inrae.fr/hal-02697404
Contributor : Migration Prodinra Connect in order to contact the contributor
Submitted on : Monday, June 1, 2020 - 11:23:46 AM
Last modification on : Monday, September 20, 2021 - 4:10:14 PM

Links full text

Identifiers

Collections

Citation

Philippe Langella, Jean-Christophe Piard, Isabelle Poquet, Yves Le Loir, Alexandra Gruss. Exportation de protéines hétérologues chez Lactococcus lactis : développement d'outils moléculaires et applications. Sciences des Aliments, Lavoisier Abonnements, 2000, 20 (1), pp.135-142. ⟨10.3166/sda.20.135-142⟩. ⟨hal-02697404⟩

Share

Metrics

Record views

20