Isolation, sequencing and analysis of the expression of Bryonia calmodulin after mechanical perturbation - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Plant Molecular Biology Year : 1993

Isolation, sequencing and analysis of the expression of Bryonia calmodulin after mechanical perturbation

Abstract

A cDNA clone (Bc329) encoding calmodulin was isolated from a Bryonia cDNA library by screening with cloned Arabidopsis calmodulin cDNA. The cDNA Bc329 was 899 bp full-length clone. The predicted amino acid sequence consists of 149 residues and reveals a high homology with other known plant calmodulins (91 to 99% identity). Genomic southern blot suggests that Bryonia calmodulin is encoded by a single-copy gene. The Bc329 clone was used as a probe to study the expression of calmodulin mRNA after a mechanical stimulus applied on young Bryonia internodes. The steady-state of calmodulin mRNA reached a maximum 30 min after the treatment before it progressively decreased. The role of calcium and calmodulin as second messengers is discussed with regard to environmental changes.

Domains

Vegetal Biology

Dates and versions

hal-02713018 , version 1 (01-06-2020)

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Jean-Philippe Galaud, Jean-Jacques Lareyre, Nicole Boyer. Isolation, sequencing and analysis of the expression of Bryonia calmodulin after mechanical perturbation. Plant Molecular Biology, 1993, 23 (4), pp.839-846. ⟨10.1007/BF00021538⟩. ⟨hal-02713018⟩
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