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Isolation, sequencing and analysis of the expression of Bryonia calmodulin after mechanical perturbation

Abstract : A cDNA clone (Bc329) encoding calmodulin was isolated from a Bryonia cDNA library by screening with cloned Arabidopsis calmodulin cDNA. The cDNA Bc329 was 899 bp full-length clone. The predicted amino acid sequence consists of 149 residues and reveals a high homology with other known plant calmodulins (91 to 99% identity). Genomic southern blot suggests that Bryonia calmodulin is encoded by a single-copy gene. The Bc329 clone was used as a probe to study the expression of calmodulin mRNA after a mechanical stimulus applied on young Bryonia internodes. The steady-state of calmodulin mRNA reached a maximum 30 min after the treatment before it progressively decreased. The role of calcium and calmodulin as second messengers is discussed with regard to environmental changes.
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https://hal.inrae.fr/hal-02713018
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Submitted on : Monday, June 1, 2020 - 6:36:19 PM
Last modification on : Thursday, December 10, 2020 - 6:46:03 PM

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Jean-Philippe Galaud, Jean-Jacques Lareyre, Nicole Boyer. Isolation, sequencing and analysis of the expression of Bryonia calmodulin after mechanical perturbation. Plant Molecular Biology, Springer Verlag (Germany), 1993, 23 (4), pp.839-846. ⟨10.1007/BF00021538⟩. ⟨hal-02713018⟩

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