Skip to Main content Skip to Navigation
Journal articles

Ecology of microbial saprophytes and pathogens in tissue culture and field-grown plants: reasons for contamination problems in vitro

Abstract : This review compares published surveys of microbial populations in plant tissue and cell cultures with the microbial saprophytes and pathogens found on field grown plants and microbial populations in the laboratory environment. From this comparison and the measured reduction in contamination after improvements in working practices in the laboratory, conclusions can be drawn about the importance of the explant and the laboratory as sources of contamination. Mechanisms of pathogenicity in vitro are described to explain why bacteria, fungi, and yeasts that are not pathogenic to plants in the field become pathogens in plant tissue cultures. Conversely, plant metabolism and its effect on the tissue culture environment are described to explain why prokaryotes, viruses, and viroids that cause disease in the field can stay latent in vitro. Detection methods for latent contaminants in plant tissue cultures are summarized, and the strategies and methods for prevention or treatment of contamination are discussed.
Mots-clés : ECOLOGIE
Complete list of metadata

https://hal.inrae.fr/hal-02713211
Contributor : Migration Prodinra <>
Submitted on : Monday, June 1, 2020 - 6:45:23 PM
Last modification on : Friday, February 5, 2021 - 4:10:13 AM

Licence


Distributed under a Creative Commons Attribution - ShareAlike 4.0 International License

Identifiers

Collections

Citation

C. Leifert, Cindy E. Morris, W.M. Waites. Ecology of microbial saprophytes and pathogens in tissue culture and field-grown plants: reasons for contamination problems in vitro. Critical Reviews in Plant Sciences, Taylor & Francis, 1994, 13 (2), pp.139-183. ⟨10.1080/07352689409701912⟩. ⟨hal-02713211⟩

Share

Metrics

Record views

16