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Description de la diversite genetique chez Brassica oleracea a l'aide de marqueurs isoenzymatiques et moleculaires

Abstract : The aim of this work is to construct homogeneous genetic pools for the multiplication of a crop collection of Brassica oleracea. Two methods of analysis of genetic diversity were compared. For intra-population investigations, the isoenzymatic method is more efficient. However, for studying population structure, the information given by RAPD (randomly amplified DNA sequence) is better correlated with morphological and agronomical diversity and is less expensive. The suggested method involves testing for the presence or absence of about 100 RAPD markers in a sample of mixed DNA from 40 seeds. The appropriate structure for the collection can be determined from inter-population and inter-cultigroup distances estimated by this technique. The data allow the construction of phylogenetic trees by the PA UP software (phylogenetic analysis using parsimony).
Keywords : RAPD
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  • HAL Id : hal-02713540, version 1
  • PRODINRA : 107469

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E. Margale, Anne-Marie Chèvre, Régine Delourme, Y. Herve. Description de la diversite genetique chez Brassica oleracea a l'aide de marqueurs isoenzymatiques et moleculaires. Genetics Selection Evolution, BioMed Central, 1994, 26 (suppl1), pp.137s-153s. ⟨hal-02713540⟩

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