Skip to Main content Skip to Navigation
Journal articles

Characterization of the virA virulence gene of the nopaline plasmid, pTiC58, of Agrobacterium tumefaciens

Abstract : We have determined the complete nucleotide sequence of a 4.8 kilobase fragment encompassing the virA locus of the nopaline-type plasmid, pTiC58, of Agrobacterium tumefaciens. virA is composed of a single open reading frame of 2499 nucleotides, capable of encoding a protein of 91.3 kiloDaltons. A trpE::virA gene fusion was used to confirm the reading frame of virA. High nucleotide and amino acid sequence homologies were observed between pTiC58 virA and the virA sequences of three octopine-type plasmids. Strong homologies in amino acid sequence were observed between pTiC58 VirA and seven bacterial proteins which control various regulons. Two hydrophobic domains within VirA are also consistent with a model in which VirA acts as a membrane-bound sensor of plant signal molecules.
Document type :
Journal articles
Complete list of metadata

https://hal.inrae.fr/hal-02727766
Contributor : Migration Prodinra <>
Submitted on : Tuesday, June 2, 2020 - 3:22:00 AM
Last modification on : Thursday, April 8, 2021 - 5:10:02 PM

Identifiers

Collections

Citation

Patrice Morel, B. S. Powell, Peter Rogowsky, C. I. Kado. Characterization of the virA virulence gene of the nopaline plasmid, pTiC58, of Agrobacterium tumefaciens. Molecular Microbiology, Wiley, 1989, 3 (9), pp.1237-1246. ⟨10.1111/j.1365-2958.1989.tb00274.x⟩. ⟨hal-02727766⟩

Share

Metrics

Record views

14