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Determination of the molecular size of the 5-HT3 receptor binding site by radiation inactivation.

Abstract : The radiation inactivation technique has been used to estimate the molecular size of the 5-HT3 receptor binding site labelled by [3H]zacopride, in comparison with that of the 5-HT1A receptor binding site labelled by [3H]8-OH-DPAT, in rat cortical membranes. The calculated molecular weight of the 5-HT3 site: 35.4 +/- 2.2 kDa (mean +/- S.E.M., n = 4) was significantly less than that of the 5-HT1A site: 62.9 +/- 1.8 kDa (mean +/- S.E.M., n = 4) and of other 5-HT1 and 5-HT2 receptors of the G-protein coupled family. These data further support that the 5-HT3 receptor is not coupled to G-proteins in the rat brain.
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https://hal.inrae.fr/hal-02727964
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Submitted on : Tuesday, June 2, 2020 - 3:30:28 AM
Last modification on : Wednesday, June 16, 2021 - 11:46:04 AM

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Henry Gozlan, L E Schechter, Francisco Bolaños-Jiménez, M B Emerit, M C Miquel, et al.. Determination of the molecular size of the 5-HT3 receptor binding site by radiation inactivation.. European Journal of Pharmacology, Elsevier, 1989, 172 (6), pp.497-500. ⟨10.1016/0922-4106(89)90033-3⟩. ⟨hal-02727964⟩

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