Plant viruses transmitted by insects cause tremendous losses in most important crops around the world. The identification of receptors of plant viruses within their insect vectors is a key challenge to understanding the mechanisms of transmission, and offers an avenue for future alternative control strategies to limit viral spread. Most plant viruses transmitted by insect vectors bind to their mouthparts on the cuticle lining the inner face of maxillary stylets. Viruses are acquired and inoculated within seconds when insects move from plant to plant. The receptors involved remains totally elusive due to long-standing technical bottleneck in working with insect cuticle. Over the last ten years, we have discovered the acrostyle, an organ located at the distal tip of aphid maxillary stylets shown to harbor receptors of a non-circulative virus, the Cauliflower mosaic virus (CaMV). Using an antibody library specifically targeting cuticular proteins, we identified very recently the two first proteins of the acrostyle, Stylin-01 and Stylin-02. These two proteins belong to the CPR family, and are highly conserved among aphid species. We further showed that one peptide of these proteins emerges all over the surface of the acrostyle, at the virus-vector interface. We developed complementary approaches –in vitro competition assays and in vivo functional validation– to assess their involvement in the virus transmission process. Our results indicate that one of the two Stylins identified plays a role in CaMV transmission.