The pesticide fate in soil is currently studied through laboratory experiments, using homogenized soil and controlled incubation conditions. However, the representativeness of such experiments is questionable regarding to field conditions, i.e., soil heterogeneity, vegetation cover and climatic variations. Upon the few studies performed simultaneously under field and laboratory, the pesticides persistence and/or sorption on soil were evaluated as different; thus bioavailability could also be different. This study aimed at comparing the dissipation and the bioavailability of dimoxystrobin and epoxiconazole (two fungicides used in the commercial formulation of Swing Gold®) in a loamy soil and in earthworms under laboratory and field conditions. Field experiments were conducted in a meadow located in Versailles (France) on 100 m2 plots. The field soil and earthworms were regularly sampled after the treatment, over one year (April to April). Laboratory experiments were performed by mixing the fungicide solution with surface soil issuing from the same field, and incubated in dark, at 15°C and constant humidity. In both cases, four replicates were performed using the recommended Swing Gold® dose, extra doses adapted to observe ecotoxicological effects and controls. The dimoxystrobin and epoxiconazole concentrations in soil were determined by an exhaustive extraction method and, to evaluate their availability, with a mild method engaging hydroxypropyl-β-cyclodextrin. At the same time, the bioavailability of the two fungicides was evaluated by determining their concentrations in exposed earthworms Aporrectodea icterica and Aporrectodea caliginosa. All analyses were performed by UHPLC-MS/MS. Under field conditions and five days after pesticide application, only 10% to 45% of pesticide residues were measured in topsoil, with high heterogeneity between replicates. After one month, the concentrations in soil increased, probably due to a plant-soil transfer. By contrast, applied dose was observed at initial time under laboratory conditions. For later dates and in both cases, dissipation was observed. The available fraction showed homogeneous rates under controlled conditions but highly heterogeneous ones in the field. However, the ratio of available/total concentrations showed the same trend of fate for the two tested substances. The bioavailability of the two fungicides was also different between field and laboratory conditions in terms of heterogeneity.