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Poster

Synchrotron label free multimodal imaging to explore lipid droplets in living cells

Abstract : Imaging native intracellular compartments in living cells is still challenging. The use of tagged proteins often modifies the morphology and the smooth running of the organelles. Methods based on intrinsic fluorescence of molecules upon excitation by deep-UV illumination are emerging for living cell imaging. We used deep UV light from synchrotron SOLEIL DISCO beamline to settle up and perform protein absorption imaging on single living yeasts. This technique was combined with transmission fluorescence microscopy. The visualization of contrasted signals inside the cells revealed chemical heterogeneity at the subcellular level. Fluorescence microscopy showed organelles with low fluorescence after 270-nm excitation. We could discriminate two populations, with high or low absorbance after 270-nm excitation. The first population was identified as lipid droplet using TMA-DPH and DPH lipid stains. Surprisingly, the second population corresponds to vacuoles. Combining deep UV fluorescence and absorption, two emerging techniques, enabled the acquisition of information on both organelle organization and chemical composition.
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https://hal.inrae.fr/hal-02736520
Déposant : Migration Prodinra <>
Soumis le : mardi 2 juin 2020 - 17:53:23
Dernière modification le : samedi 27 juin 2020 - 19:44:07

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  • HAL Id : hal-02736520, version 1
  • PRODINRA : 435717

Citation

Frederic Jamme, Isabelle Bouchez, Caroline Pénicaud, Stéphanie Passot, Fernanda Fonseca, et al.. Synchrotron label free multimodal imaging to explore lipid droplets in living cells. Conférence Jacques Monod Analyses moléculaires de l’organisation et du remodelage des membranes, Apr 2017, Roscoff, France. 2017. ⟨hal-02736520⟩

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